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Leadgene Leadgene
Swine IL-15, His Tag, E. coli
Catalog Number

LDG015PSE

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  • Specifications
    • Species of Origin

      Swine

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 14.06 kDa.
      The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in TF-1 cells.
      The ED₅₀ for this effect is <5.5 ng/mL.

      Endotoxin level

      <0.01 EU per 1 µg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 15 (IL-15) is a 12.65 kDa cytokine with 114 amino acid residues and is primarily produced from mononuclear phagocytes like dendritic cells, monocytes, and macrophages. IL-15 plays a critical role in the innate immune system when IL-15 binds to the IL-15 receptor. In addition, IL-15 mediates biological functions, such as the proliferation of natural killer cells and T cells, preventing the immune cells from apoptosis by activating STAT3 /JAK and PI3K/ALT signaling pathways.

      Synonyms

      Interleukin-15, IL-15

    • Uniprot ID

      #Q95253

      Sequence Note

      Thr49-Ser162

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant swine IL-15.

    SDS-PAGE analysis of recombinant swine IL-15.

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  • Datasheet & Documents
    MSDS
    Manual
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