Description
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 5’→3’ synthesis of RNA from DNA downstream from its promoter.
Components
25,000 U
Items
T7 RNA Polymerase (200 U/μL)
Quantity
1 vial (25,000 U)
Items
10× RNA Polymerase reaction buffer
Quantity
1 vial (1 mL)
Items
100 mM DTT
Quantity
1 vial (1 mL)
Expression system
Escherichia coli
Buffer
T7 RNA Polymerase is supplied in 100 mM Tris-HCl (pH 7.9), 20 mM KCl, 1 mM DTT, 1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) glycerol.
Purity
>98% as determined by SDS-PAGE analysis.
Unit Definition
One unit is defined as the amount of the enzyme incorporates 1 nmol of ATP into acid-insoluble product in 1 hour at 37°C.
Reaction Condition
1× RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
10× RNA Polymerase Reaction Buffer: 400 mM Tris-HCl (pH 8.0), 60 mM MgCl₂, and 20 mM spermidine.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
SDS-PAGE analysis of recombinant T7 RNA Polymerase.
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Disclaimer：For Research Use or Further Manufacturing Only.
