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Species of Origin
Streptococcus pyogenes
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 159.27 kDa.
The protein migrates as 130-180 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
The wtCas9 is a recombinant Streptococcus pyogenes Cas9 protein (wild type form) purified from E. coli. Cas9 protein can form a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA). The RNP complex can recognizes the target site by matching gRNA with the genomic DNA sequence and make DNA breaks within 3 bases from the NGG PAM. With the CRISPR/Cas9 system, researchers can valid gene editing assay in cells or other kinds of animals.
Synonyms
CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9
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Uniprot ID
#Q99ZW2
Sequence Note
Met1-Asp1368
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant wtCas9.]()
SDS-PAGE analysis of recombinant wtCas9.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
