Antibody Humanization Service

Antibody humanization is a critical process of therapeutic antibody development, which can reduce the immunogenicity of monoclonal antibodies derived from xenogeneic sources lead to rejection reaction like HAMA (human anti-mouse antibody response) by replacing non-human antibody structures with human ones.

Leadgene offers the finest antibody humanization service for your drug development.

Our dedicated project manager will support and discuss with you personally throughout the process that help you obtain the ideal humanized antibodies.

• Key service features:
  • Our dedicated team will provide the most professional and attentive sequence prediction of the humanized antibody.
  • Our antibody humanization service features to its low back mutation (usually lower than six amino acids) that can reduce the immunogenicity of
  antibody in clinical application.
  • We offer Biacore T200 service for clients that help you understand the binding affinity of your humanized antibody. (clients need to provide antigen)
  We will provide small amount of humanized antibody for you.
• Service workflow:
  • Clients need to provide the sequence of monoclonal antibody variable region.
  • Clients need to offer the information of your interested antibody.
  • The gene structure of antibody VH and VL sequence will be analyzed by our experts.
  • Chimeric antibody production, which contain mouse V region and human C region.
  • Correct the sequence of humanized antibody with matching to human germline template.
  • We will design and produce multiple candidates of humanized antibodies.
  • We will pick up the best clone of these candidates depend on the test results.
• Sample requirements:
  • Clients need to provide the sequence of monoclonal antibody variable region.
  • Clients should offer sufficient antigen for antibody screening and Biacore antibody affinity analysis.
• Deliverables:
  • Final report (including sequence analysis, results of antibody screening and Biacore affinity test).
  • Plasmid for expressing humanized antibody. (heavy chain*1, light chain*1)
     We will provide small amount of humanized antibody for you.