Polyclonal Antibody Development

Leadgene has amassed several years of significant expertise in antibody development and production.

We offer professional mouse and rabbit immunization and polyclonal antibody production services tailored to meet our clients' needs.

Our customized services deliver comprehensive support for all stages of polyclonal antibody production, spanning from immunogen design and synthesis to antibody production and purification.

These processes ultimately enhance the specificity and affinity of the antibodies produced.

• Key service features:
  • Our experts will offer professional analysis and suggestion for antigen design.
  • Our capable project manager and scientists will support and discuss with you personally throughout the process.
  • Flexibility to increase the number of immunized animals.
  • We provide professional affinity purification technology to increase the purity of antibody.
• Service workflow:
  • Antigen design and evaluation
  • Carrier protein-conjugated peptide synthesis or customized protein production (depend on clients’ requirements).
  • Four mouse immunization with your target antigen.
  • Collecting the serum from each of antigen-immunized mouse.
  • Improving the specificity and affinity of antibody by affinity purification.
  • Binding ability test of antibody to immunogen by Indirect ELISA test and QC report.
• Sample requirements:
  • Clients should offer the sequence for their target peptide or protein immunogen.
  • If clients prefer to use their own peptide as immunogen, the following condition should be complied.
  Peptide: concentration ≧ 1 mg/mL, Amount ≧ 5 mg, Purity > 85%.
  * To confirm the peptide quality, client should provide the HPLC result, MS data and sequence of peptide)
  * For client who want to do affinity purification, more than 5 mg of peptide should be provided. (usually needs about 8-10 mg).
  • If clients prefer to use their own protein as immunogen, the following condition should be complied.
  Protein: concentration ≧ 1 mg/mL, Amount ≧ 3~5 mg, Purity > 90%
  * To confirm the protein quality, client should provide the SDS-PAGE, QC data.
  * For client who want to do affinity purification, more than 10 mg of protein should be provided.
• Deliverables:
  • Post-immunized serum.
  • Indirect ELISA test of antibody to immunogen and QC report of purified antibody with or without immunization, titer > 1:50,000.

• Key service features:
  • Our experts will offer professional analysis and suggestion for antigen design.
  • Our capable project manager and scientists will support and discuss with you throughout the process.
  • Flexibility to increase the number of immunized animals.
  • We provide professional affinity purification technique to increase the purity of antibodies.
• Service workflow:
  • Antigen design and evaluation
  • Carrier protein-conjugated peptide synthesis or customized protein production (depending on clients’ requirements)
  • Two New Zealand Rabbit immunization with your target antigen
  • Collecting the serum from each of antigen-immunized rabbit
  • Improving the specificity and affinity of antibodies by affinity purification
  • Binding ability test of antibody to immunogen by indirect ELISA test and QC report
• Sample requirements:
  • Clients should offer the sequence for their target peptide or protein immunogen.
  • If clients prefer to use their own peptides as immunogens, the following condition should have complied.
  Peptide: concentration ≧ 1 mg/mL, Amount ≧ 5 mg, Purity > 85%.
  * To confirm the peptide quality, the client should provide the HPLC result, MS data, and sequence of the peptides.
  * If the clients want to do an affinity purification, they need to provide at least 5 mg of peptides. (We usually need 8-10 mg.)
  • If clients prefer to use their own proteins as immunogens, the following condition should have complied.
  Protein: concentration ≧ 1 mg/mL, Amount ≧ 3~5 mg, Purity > 90%
  * To confirm the protein quality, the client should provide the SDS-PAGE, QC data.
  * If the clients want to do an affinity purification, they need to provide at least 10 mg of proteins.
• Deliverables:
  • 1 mg peptide or X mg protein immunogen (depend on client requirement)
  • 2-10 mg purified rabbit polyclonal antibody
  • Indirect ELISA test of antibody to the immunogen

• Citation:
  Wu CH, Wu MK, Lu CC, Tsai HP, Lu YY, Lin CL. Impact of Hepatoma-Derived Growth Factor Blockade on Resiniferatoxin-Induced Neuropathy. Neural Plast. 2021.